Working to understand: temporal lobe epilepsy
Scientific Focus: My laboratory used a rat model of temporal lobe epilepsy that involves injecting a chemoconvulsant, pilocarpine. The main region of the brain affected in this type of epilepsy is the hippocampus. I have contributed in recent years by showing newly generated neurons in the dentate gyrus of the adult hippocampus form basal dendrites that are involved in recurrent excitatory circuitry. This type of circuitry may be the basis for seizure activity in these rats. Recently, I have examined the role of neuroglia in epilepsy and discovered a new type of neuronal death involving microglia. This form of neuronal death occurs in brain regions where adult neurogenesis is found and thus may be involved in the homeostasis of the number of neurons in these brain regions.
Seizure-induced formation of basal dendrites on granule cells of the rodent dentate gyrus.
, Shapiro LA, Yan XX, Dashtipour K, Nadler JV, Obenaus A, Spigelman I, Buckmaster PS. In: Noebels JL, Avoli M, Rogawski MA, Olsen RW, Delgado-Escueta AV, editors.
Jasper's Basic Mechanisms of the Epilepsies [Internet]. 4th edition. Bethesda (MD): National Center for Biotechnology Information (US); 2012.
Synaptic connections of hilar basal dendrites of dentate granule cells in a neonatal hypoxia model of epilepsy.
Sanchez RM, Shapiro LA.
Epilepsia. 2012 Jun;53 Suppl 1:98-108.
Astrocyte Alterations in the Hippocampus Following Pilocarpine-induced Seizures in Aged Rats.
Arisi GM, Ruch M, Foresti ML, Mukherjee S,Shapiro LA.
Aging Dis. 2011 Aug;2(4):294-300. Epub 2011 Aug 30.
Seizure-induced Increased Neurogenesis Occurs in the Dentate Gyrus of Aged Sprague-Dawley Rats.
Shapiro LA, Wang L, Upadhyaya P,
Aging Dis. 2011 Aug;2(4):286-93. Epub 2011 Aug 30.
Morphological and ultrastructural features of Iba1-immunolabeled microglial cells in the hippocampal dentate gyrus.
Shapiro, L.A., Perez, Z.D., Foresti, M.L., Arisi, G.M. and
Brain Res. 1266: 29-36 (2009). Cover Photo-micrograph.
Proximity of excitatory and inhibitory axon terminals adjacent to pyramidal cell bodies provides a putative basis for nonsynaptic interactions. Merchán-Pérez, A., Rodriguez, J.R., and DeFelipe, J.
Proc. Natl. Acad. Sci. USA, 106: 9878-9883 (2009).
Neurons born in the adult dentate gyrus form functional synapses with target cells.
Toni N, Laplagne DA, Zhao C, Lombardi G,, Gage FH, Schinder AF.
Nat Neurosci. 2008 Aug;11(8):901-7. Epub 2008 Jul 11.
In DRG11 knock-out mice, trigeminal cell death is extensive and does not account for failed brainstem patterning. </
Jacquin MF, Arends JJ, Xiang C, Shapiro LA,, Chen ZF.
J Neurosci. 2008 Apr 2;28(14):3577-85.
Dep't Anatomy & Neurobiology, School of Medicine,
University of California,
Irvine, CA 92697-1280
Donations to labs may be applied in many ways: from costs for experiments to support for a student's stipend.
Join us in becoming a part of scientific history, by supporting the work of this lab.
Learn more by contacting Sara Johnson, email@example.com, or by visiting our Get Involved Page, where you can donate online.