Working to understand: temporal lobe epilepsy
Scientific Focus: My laboratory uses a rat model of temporal lobe epilepsy that involves injecting a chemoconvulsant, pilocarpine. The main region of the brain affected in this type of epilepsy is the hippocampus. I have contributed in recent years by showing newly generated neurons in the dentate gyrus of the adult hippocampus form basal dendrites that are involved in recurrent excitatory circuitry. This type of circuitry may be the basis for seizure activity in these rats. Recently, I have examined the role of neuroglia in epilepsy and discovered a new type of neuronal death involving microglia. This form of neuronal death occurs in brain regions where adult neurogenesis is found and thus may be involved in the homeostasis of the number of neurons in these brain regions.
Brain Structure & Function, 214:25-35 (2009).Shapiro, L.A., Perez, Z.D. and Spigelman, I., Microglia-associated granule cell death in the normal adult dentate gyrus.
Shapiro, L.A., Perez, Z.D., Foresti, M.L., Arisi, G.M. and Brain Res. 1266: 29-36 (2009). Cover Photo-micrograph.Morphological and ultrastructural features of Iba1-immunolabeled microglial cells in the hippocampal dentate gyrus.
Díaz-Cintra, S., Xue, B., Spigelman, I., Van, K., Wong, A.M., Obenaus, A. and Brain Res. 1285: 182-187 (2009).Dentate granule cells form hilar basal dendrites in a rat model of hypoxia-ischemia.
Merchán-Pérez, A., Rodriguez, J.R., Proc. Natl. Acad. Sci. USA, 106: 9878-9883 (2009).and DeFelipe, J., Proximity of excitatory and inhibitory axon terminals adjacent to pyramidal cell bodies provides a putative basis for nonsynaptic interactions.
Sultan-Styne, K., Toledo, R., Walker, C., Kallkopf, A., J. Comp. Neurol. 515: 696-710 (2009).and Guthrie, K.M., Long-term survival of olfactory sensory neurons after target depletion.
Shapiro LA, Ng K, Zhou QY,
Subventricular zone-derived, newly generated neurons populate several olfactory and limbic forebrain regions. Epilepsy Behav. 2009 Jan;14 Suppl 1:74-80. Epub 2008 Oct 26.
Structural changes for adult-born dentate granule cells after status epilepticus. Epilepsia. 2008 Jun;49 Suppl 5:13-8. Review.
Toni N, Laplagne DA, Zhao C, Lombardi G,
Neurons born in the adult dentate gyrus form functional synapses with target cells. Nat Neurosci. 2008 Aug;11(8):901-7. Epub 2008 Jul 11.
Synaptic input to dentate granule cell basal dendrites in a rat model of temporal lobe epilepsy. J Comp Neurol. 2008 Jul 10;509(2):190-202.
Jacquin MF, Arends JJ, Xiang C, Shapiro LA,
In DRG11 knock-out mice, trigeminal cell death is extensive and does not account for failed brainstem patterning. J Neurosci. 2008 Apr 2;28(14):3577-85.
Shapiro LA, Wang L,
Rapid astrocyte and microglial activation following pilocarpine-induced seizures in rats. Epilepsia. 2008;49 Suppl 2:33-41.
Dep't Anatomy & Neurobiology, School of Medicine,
University of California,
Irvine, CA 92697-1275
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